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KMID : 0364820080440010022
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Korean Journal of Microbiology
2008 Volume.44 No. 1 p.22 ~ p.28
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Biodegradation of Cutting Oil by Pseudomonas aeruginosa KS47
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Kim Lan-Hee
Lee Sang-Seob
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Abstract
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Cutting oils are emulsionable fluids widely used in metal working processes. Their composition is mineral oil, water, and additives (fatty acids, surfactants, biocides, etc.) generating a toxic waste after a long use. Cutting oils also affect colour, taste and odour of water, making it undesirable for domestic and industrial uses. In these days, conventional treatment methods as evaporation, membrane separation or chemical separation have major disadvantages since they generate a concentrated stream that is more harmful than the original waste. In this study, our purpose is to reduce cutting oils by using biological treatment. Eighty one strains were isolated from cutting waste oil of industrial waste water sludge under aerobic conditions. Among these strains, KS47, which removed 90.4% cutting oil in 48 hr, was obtained by screening test under aerobic conditions (pH 7, 28oC). KS47 was identified as Pseudomonas aeruginosa according to morphological, physiological and biochemical properties, 16S rDNA sequence, and fatty acid analysis. P. aeruginosa KS47 could utilize cutting oil as carbon source. In batch test, we obtained optimal degradation conditions (1.5 g/L cell concentration, pH 7, and temperature 30oC). Under the optimal conditions, 1,060 mg/L cutting oil was removed 83.7% (74.1 mg/L/hr).
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KEYWORD
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biodegradation, cutting oil, Pseudomonas aeruginosa KS47
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